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| A technique for quantitative cytology of nasal secretions.
Lee HS, Majima Y, Sakakura Y, Kim BW.
Eur
Arch Otorhinolaryngol. 1991;248(7):406-8.
Department of Otorhinolaryngology, Mie University School
of Medicine, Japan. |
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To quantitatively analyze cellular elements in mucopurulent
nasal secretions from patients with chronic paranasal sinusitis,
we studied five mucus-liquefying agents--dithiothreitol (DTT),
ethylenediaminetetraacetic acid tetrasodium salt, propylene
glycol, serratio peptidase and urea--with Hanks' balanced
salt solution used as a control. Agents were compared for
the number of cells, the mucus-liquefying effect and the
staining characteristic of cells in cytocentrifuge-prepared
smears. The results indicated that the treatment of nasal
secretions with 10 mM DTT provides more clear and detailed
cytological preparations for quantitative cytology of nasal
secretions. |
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